List of Contributions Conference MendelNet 2015

Plant Production

THE YIELD AND QUALITY OF WINTER WHEAT (TRITICUM AESTIVUM) GRAIN AFTER APPLICATION OF MICRNUTRIENTS ON SEED
ANTOSOVSKY JIRI, RYANT PAVEL
view abstract fulltext xantoso6@mendelu.cz

Abstract: Fertilization with microelements in a crop production is not generally important until deficiency symptoms appeared on plants. Soil application is expensive and foliar application may not remove a deposit of microelements. Seed coating could be more economical way. Seeds with microelements should be more complex and should provide enough nutrients, especially for the first stage of growth and development. In the experiment, seeds were coated by manganese, copper, zinc, molybdenum and by combination of Mn-Zn-Cu. The same fertilizers were used as foliar nutrition. There was default fertilization with nitrogen for all variants. The control observation was microelements free. The results showed no statistically difference between control variant and seed coating or foliar nutrition in any category (value of N-tester, yield or grain quality). On the other side, there were no deficiency symptoms on plants. Contents of micronutrients in leafs were slightly higher than the control observation. Seed coating with micronutrients has fulfilled its preventive purpose.
VARIABILITY OF THE ESSENTIAL OIL CONTENT IN LAVENDER (LAVANDULA ANGUSTIFOLIA P. MILL.)
BOSKO RASTISLAV, PLUHACKOVA HELENA, SVOBODA ZDENEK
view abstract fulltext rastislav.bosko@gmail.com

Abstract: The aim of this work was the determination of variability of the essential oil content in lavender obtained from different sources. The results indicate that significant differences were found both in the essential oil content and in its composition. The essential oil content varied in the range of 2.10–2.83 ml.100 g^-1. Thus, it is important to distinguish individual lavender types according their usage. The Czech Pharmacopoeia (2009) states that the lavender should contain 13 ml.kg^-1 of the essential oil.
SPECIES SPECTRUM OF VEGETATION ON SELECTED SECTIONS OF RAILWAY
CERVENKOVA JANA, WINKLER JAN
view abstract fulltext xcerven3@node.mendelu.cz

Abstract: This paper focuses on the evaluation of weed species composition on the selected railway sections between the cities Chrudim and Úhřetice. Weed species spectrum was evaluated according to phytocoenology relevé. Eleven phytocoenology relevé were carried out in two periods, the first in July and the second in August 2013. The obtained data were processed by multivariate analysis of ecological data, segment analysis DCA (Detrended Correspondence Analysis) and Canonical Correspondence Analysis (CCA). 85 species of plants were found on the railway. The highest coverage had species as: Potentilla reptans, Urtica dioica, Equisetum arvense, Convolvulus arvensis. The highest coverage on utilized railway had species Equisetum arvense, Urtica dioica, Potentilla reptans, Convolvulus arvensis. Species as Potentilla reptans, Clematis vitalba, Linaria vulgaris, Senecio vulgaris, Geum urbanum had the highest coverage on unused railway.
ESTIMATION OF ABOVEGROUND BIOMASS OF CATCH CROPS USING NDVI MEASUREMENTS
HANDLIROVA MARTINA, LUKAS VOJTECH, PROCHAZKOVA BLANKA, SMUTNY VLADIMIR
view abstract fulltext martina.handlirova@mendelu.cz

Abstract: For the fulfilment of the positive effects of catch crops it is necessary to create a sufficient amount of biomass. The amount of biomass of catch crops is traditionally evaluated by destructive sampling that are labour and time consuming and may not represent the whole monitored area. Within the period of 2013–2014, the aboveground biomass of catch crops was evaluated using ground measurements of vegetation index NDVI and the results were compared with traditionally carried sampling of plant matter. The study took place on a small-plot field trial established in 2006 at the experimental field station in Žabčice (South Moravia, Czech Republic). The experiment included nine kinds of catch crops. Catch crops growths were set up after every harvest of winter wheat (in mid-August). The experiment included control variant without catch crop sowing. Regression analysis for both years of monitoring showed positive dependence between the amount of vegetable matter (fresh and dry matter) and vegetation index NDVI. The accuracy of the measurement depends on the state of growths, particularly with regard to the lower sensitivity of the NDVI when a certain degree of leaf area index is reached. Despite these shortcomings, the spectral measurement is a good alternative to traditional methods, mainly due to rapid and simple measurement and its easy repeatability without damaging the crop.
PRODUCTION CAPABILITIES OF CATCH CROPS AND THEIR IMPACT ON THE GRAIN YIELD OF SPRING BARLEY
HANDLIROVA MARTINA, PROCHAZKOVA BLANKA, SMUTNY VLADIMIR
view abstract fulltext martina.handlirova@mendelu.cz

Abstract: Catch crops are grown between two main crops, their significance is multifaceted. Catch crops may affect the subsequent crop. The aim of this study was to evaluate the production capabilities of catch crops in the years 2011–2014 and their impact on the grain yield of spring barley in the years 2012–2015. The field experiment was set up in an experimental field station in Žabčice, South Moravia, Czech Republic. The experiment included ten kinds of catch crops: Sinapis alba, Raphanus sativus v. oleifera, Phacelia tanacetifolia, Fagopyrum esculentum, Secale cereale v. multicaule, Panicum miliaceum, Crambe abyssinica, Malva verticillata, Phalaris canariensis, Carthamus tinctorius. The experiment also included a control variant - without catch crops. Catch crop growths were set up after winter wheat. Catch crops were left on the lot until spring. The subsequent crop after catch crop was spring barley. Weather conditions affected the production capabilities of catch crops. From the point of view of securing the purpose of growing catch crops, it is necessary to include Sinapis alba, Raphanus sativus v. oleifera, Crambe abyssinica and Phacelia tanacetifolia, which reached regularly the highest yields. When there is enough water in the winter and spring, catch crops have no negative impact on yields of spring barley.
EFFECT OF TEMPERATURE STRESS AND WATER SHORTAGE ON THOUSAND GRAIN WEIGHT OF SELECTED WINTER WHEAT VARIETIES
HLAVACOVA MARCELA, POHANKOVA EVA, KLEM KAREL, TRNKA MIROSLAV
view abstract fulltext marci.hlava.22@gmail.com

Abstract: The aim of the presented study was to assess the effect of high temperatures and water shortage during anthesis on thousand grain weight (TGW) of two winter wheat varieties (Tobak and Pannonia). In addition, numbers of grains per spike were also assessed. The six growth chambers were used to simulate heat stress conditions within following gradient of temperature maxima: 26°C (control chamber), 29, 32, 35, 38 and 41°C. The relative humidity (RH) course and photosynthetically active radiation (PAR) intensity were controlled via protocols. Additionally, drought stressed (dry) and well-watered (wet) treatments were established within each growth chamber. The plants were removed from the growth chambers after 14 days and they were left until a full maturity, exposed to actual weather conditions. The TGW at 14% moisture were evaluated for particular treatments within both winter wheat varieties. TGW was generally more affected by high temperatures under drought stress than in well-watered conditions. The results revealed that Pannonia TGW was much more affected by the water deficiency in combination with high temperature (particularly 38 and 41°C) than Tobak TGW.
THE INFLUENCE OF FERTILIZATION AND PRESERVATION ON THE CONTENT OF MYCOTOXINS IN SILAGE OF COCKSFOOT (DACTYLIS GLOMERATA L.)
HODULIKOVA LUCIA, KVASNOVSKY MICHAL, KNOT PAVEL, KLUSONOVA IVA, NEDELNIK JAN, SKLADANKA JIRI
view abstract fulltext lucia.hodulikova@mendelu.cz

Abstract: The aim of this work was to assess the content of mycotoxins with regard to habitat and fertilization of cocksfoot crops (Dactylis glomerata L.) varieties Niva. Experimental forests were based on two sites with different altitudes. The harvest took place at the beginning of the earing (half of May) when the first cuts of the biomass were analyzed. The samples were analyzed for nutrients (ash, crude protein and crude fiber), the quality of the water extracts (ammonia, ethanol, pH, lactic acid, acetic acid) were evaluated, and the contents of mycotoxins (deoxynivalenol – DON, zearalenone – ZEA) were determined in green mass of silage. The data was assessed by analysis of variance ANOVA P < 0.05 on the surface. Fertilization did not influence the content of the organic nutrients in the silages. The difference (P < 0.05) in the quality of the silage extracts made from fertilized and unfertilized biomass, higher (P < 0.05) acetic acid content and a lower content of ethanol were noticeable. There was also noticeable higher content of ZEA in unfertilized stands. Silage did not show increased content of mycotoxins. The evaluation of the content of organic nutrients and acids proved that the quality of silage is very good.
HOW SEEDING PASTURES AFFECT NITROGEN SUBSTANCES CONTENT IN THE FORAGE
HORTOVA MAGDALENA, SKLADANKA JIRI, NOVOTNA MONIKA, HLOUCALOVA PAVLINA
view abstract magdalena.hortova@mendelu.cz

Abstract: The aim of the diploma thesis was to analyse the effect of a seeding on a quality of fodder as well as the participation of added varieties of the clover meadow and other hybrids. Sowing machines with different intensity of the original turf were chosen to be added to the seeding. These sowing machines were: GP TP–300, SE 2–024 and PP–2. The crop yield of dry basis, the content of nitrogenous substances, a fibre and NEL were evalueted. The values were specified with the aid of the method of Spektroscopie in the near infrared area (NIR Systems 6500). Participation of added species was evalueted by the method of projective dominance. The added varieties of species didn´t proved convincing difference in the content of nitrogenous substances of growth, but the content of NL was different with regard to the sequence of mowings (P<0.05). The seeding of the clover meadow had a positive influence on the content of NEL in the growth (P<0.05). I tis recommended to add a variety of Felina to a pasture for horses because it is convenient as a seeding to pasters and it has many advantages like a resistence to a trampling or good hibernation. It provides also good crop yield and thanks to its structure and its content of nutriments it is fittind to feed horses.
MONITORING OF WATER USE, DROUGHT AND YIELD IMPACTS OF WINTER WHEAT USING IMAGINERY FROM SATELLITES
JURECKA FRANTISEK, ANDERSON MARTHA, HLAVINKA PETR, SEMERADOVA DANIELA, TRNKA MIROSLAV, HAIN CHRISTOPHER, GAO FENG, YANG YUN, ZALUD ZDENEK
view abstract fulltext frantisek.jurecka@centrum.cz

Abstract: Remote sensing can be very useful tool for drought monitoring, providing valuable information about yield-limiting moisture conditions and crop response under current climate conditions. In this study the Atmosphere-land Exchange Inverse (ALEXI) model was used. The ALEXI model uses the morning surface temperature (LST) rise and provides information on the surface moisture status. In this paper correlations between yields and satellite indicators of crop water use or evapotranspiration (ET) were studied for the period 2002–2014. Correlations were studied for winter wheat at district scale in Vysočina, Jihomoravský and Olomoucký regions since winter wheat is one of the traditional and most important crops grown in these regions. The Evaporative Stress Index (ESI) was used for these correlations as an ET-based index. Time series of Pearson correlation coefficient (r) computed between ESI and winter wheat yields at district scale were analysed. Strongest correlations are associated with districts within the Southern Moravian lowlands in Jihomoravský and Olomoucký region, where frequency of occurrence of severe drought was highest over the period of record. Severe drought resulted in significant yield impacts, particularly in years 2003 and 2012. Correlations tend to be lower over the highlands districts of Vysočina and surroundings. In these districts, yields are more temperature than moisture limited and were more stable over the period of record.
DIFFERENCES IN THE COURSE OF AIR TEMPERATURE BETWEEN THE WHEAT CANOPY GROUND AND STANDARD CLIMATOLOGICAL STATION
KRCMAROVA JANA, POKORNY RADOVAN, STREDA TOMAS
view abstract fulltext jana.krcmarova@mendelu.cz

Abstract: The temperature in the ground of wheat canopy was compared with those measured on standard climatological station by regression analysis. Measurements in the wheat stand were carried out on two localities – Žabčice (site Obora and Písky) and Branišovice from year 2015. The course of temperature in the wheat stand ground differed meaningly, the length of particular winter wheat vegetation stages was different, too. The regression equations reveals the differences of standard environment and the ground of wheat canopy. These differences were dependent on the growth stage of winter wheat and experimental site. These differences can be caused by different conditions of experimental localities on results.
EFFECT OF DROUGHT ON YIELD POTENTIAL OF SELECTED GRASS SPECIES
KVASNOVSKY MICHAL, HODULIKOVA LUCIA, PECINOVA HANA, KLUSONOVA IVA, KNOT PAVEL
view abstract fulltext michal.kvasnovsky@mendelu.cz

Abstract: The main aim of this study was to evaluate the response of the production types of grasses to stress-induced reduction of normal precipitation in relation to their production characteristics and the structure of biological phytomass. The covers were established by planting of pre-grown plants of the individual grass species in the spring of 2009 in the form of a small-plot experiment in two blocks. Block A – normal precipitation mode, Block B – reduced precipitation mode consisting in roofing of 50% of the experimental area coverage by a special film with a minimum reduction of light conditions in order to drain a half of rainfall out of the area. In the crop year 2011 the annual total Rainfall was relatively lower by 14.0% (632.8 mm) than the long-term average, i.e. 736 mm. The species with the highest ability to create fodder of Dactylis glomerata significantly decreased fodder production and formation of above-ground shoots due to reduced precipitation in meadow utilization. A simile trend was also observed in the utilization in Festuca pratensis. The lowest reduction in production due to drought appeared in Lolium perenne.
BOTANICAL SURVEY AND SUCCESSIONAL CHANGES OF VEGETATION IN POOLS AFTER RESTORATION PROJECT IN WETLAND NEAR THE CISARSKA CAVE, MORAVIAN KARST
NOVAKOVA ELISKA, JIROUSEK MARTIN, MUSIL ZDENEK, STEPANKOVA PETRA
view abstract fulltext ela1993@seznam.cz

Abstract: Three shallow pools were done during the restoration project in 2012, with a goal to create a suitable habitat for competitively weak wetland species surviving on the long-term drained locality only in a seed bank. After that, the floristic and phytosociological research was done for whole area of the wetland with special attention to pools, where succession of vegetation was continuously monitored on permanent plots. In total, 101 taxa of vascular plants and bryophytes were recorded on the study site (57 of them in the permanent plots in pools), nine recorded plant speciesare endangered in the Czech Republic.Vegetation of the study site consists predominantly of tall sedges in most wet places, surrounded by abandoned drained wet meadows. Vegetation of oligotrophic water bodies quickly enveloped in dug pools. During our 2-year monitoring, continual successional change of vegetation was found, with the gradual infiltration of species from surrounding vegetation. Strong effect on the vegetation has also the fluctuating water level. We assume that in the long-term perspective, both the hydrological conditions and other restoration activities will be crucial for surviving of competitively weak endangered wetland species on the locality.
EFFECT OF PUCCINIA GRAMINIS ON COLOR RETENTION RATINGS OF LOLIUM PERENNE
NOVOTNA MONIKA, SKLADANKA JIRI
view abstract fulltext xnovot14@mendelu.cz

Abstract: The aim of this study was to evaluate the effect of Puccinia graminis on important economic characteristics. The experiment was carried out in cooperation with the Breeding Station in Větrov. The results of lawn experiments in 2014 showed that the selection of genotypes of perennial ryegrass less infected by rust was positively reflected not only in improved health but also in better overall appearance of progenies of selected plants. In November, the level of green was very intense while intensity in July and August was weaker. Statistically significant difference between the indigenous populations and the selection was evident only in August (0.008). Monitoring of rust will be extended by forage grasses and the attention will be paid to laboratory testing and artificial infection when testing on the field.
EVALUATION OF RESISTANCE OF SELECTED SPECIES OF GRASSES TO FUNGAL PATHOGENS
NOVOTNA MONIKA, SKLADANKA JIRI, DOHNAL VLASTIMIL
view abstract novomo@centrum.cz

Abstract: This graduatin thesis in quires into study and characteristics of fungi which most often invade pasture of Lolium perenne, festucoid hybrid (Festuca arundinae x Lolium multiflorum), loloid hybrid (Lolium multiflorum x Festuca pratensis) and mixed crops with Red Fescue (Festuca rubra) and Smooth Meadow – grass (Poa pratensis). Snow Mold Fungi (Monographella nivalis), Gray Snow Mold (Typhula incarnate), Red thread (Laetisaria fuciformis), Fusarium scarlatina, anthracnose disease, Rust Fungi Graminicola, barley powdery mildew (Blumeria graminis) and leaf spots are ranked among them. As indicator of the level invasion by fungi acts ergosterol. Its highest concetration was measured at the end of vegetative period at vegetation of Perrenial Ryegrass (Lolium perenne) and loloid hybrid (Lolium multiflorum x Festuce pratensis). This corresponds with subjective assessment of level of invasion by fungi which was at thes species the most intensive in this period. Next, it in quires into assessment of overall integration of vegetation and integration of sown species. Festucoid hybrid (Festuca arundinacea x Lolium multiflorum) had the least integrated vegetation and was the least integrated sown species in the year of establishing of vegetation. But on the contrary in the first useful year it demonstrated its competitive strength and resistance against unfavourable conditions and together with Perennial Ryegrass (Lolium perenne) were assessed on the best level from all the monitored species in both characteristics.
EFFECT OF NUTRIENTS DEFICIENCIES ON ROOT ARCHITECTURE AND GROWTH OF WINTER WHEAT
RATTANAPICHAI WUTTHIDA, KLEM KAREL
view abstract fulltext xrattana@node.mendelu.cz

Abstract: The study of the effects of N, P, K deficiencies on root architecture and growth was tested in phenotyping platform with winter wheat (Bohemia variety). The experiment was arranged with 4 treatments: Complete nutrient, Without N, Without P and Without K. The root were grown on the surface of vertically fixed black filter paper sheets (30x60cm), covered from both sides by black plastic sheets (PVC-P). The system was setup with a micro-irrigation channel in the top of sheets to ensure circulation of hydroponic medium as hydroponic system. Eighteen days after transplanting, we took the root images by the standard RGB digital camera. To evaluate the root architecture parameters the “SmartRoot” software was used. The results showed that nutrient deficiency had effect on root architecture of winter wheat. N deficiency increase in total seminal root and lateral root length and root/shoot ratio, while P deficiency resulted in increase of mean root diameter, total root area when compared to the control. N deficiency also decreased root and shoot dry weight and total leaf area. However, nutrient deficiency slightly decreased lateral density. There was a slight effect of K deficiency on root architecture when compared to the complete nutrient application. The increasing of leaf dry weight was related with the increased of root dry weight.
POSSIBILITIES OF BIOLOGICAL CONTROL OF SAN JOSE SCALE (DIASPIDIOTUS PERNICIOSUS)
RYCHLA KATERINA
view abstract fulltext katerina.rychla@mendelu.cz

Abstract: The San Jose scale (Diaspidiotus perniciosus) is a wide spread pest in the Czech Republic. Its harmfulness is increasing over the last years. The trees may die due to suction of the phloem. The only stage that is sensitive to treatments is a crawler that is not protected by cover. Possible control methods are limited to applications of oil based preparations in early spring in organic agriculture. We were testing different preparations suitable for organic agriculture against San Jose scale nymphs during years 2014 and 2015. In 2014 the highest efficacy was achieved with preparation Naturalis up to 85.2% but the most stable efficacy during the whole season was achieved with treatment Spintor (38.9–78.4%). In 2015 the results were not statistically significant due to very extreme temperatures during the periods of applications.
REGULATION OF VEGETATION ON LANDS WITH PHOTOVOLTAIC POWER PLANTS
ULDRIJAN DAN, CHOVANCOVA SVETLANA, WINKLER JAN
view abstract fulltext xuldrija@node.mendelu.cz

Abstract: This paper deals with the evaluation of weed species diversity on chosen land with photovoltaic power plant. At the same time, the impact of different regulatory interventions on the species diversity is observed. The area is located in South Moravia, Brno-country district in the village Unin. Evaluation of vegetation was carried out by method of phytocoenology relevé. Three different types of maintenance was performed on selected frames, without any intervention for the first one, second one with herbicide application and third one prepared by string trimmer. The obtained data were processed by multivariate analysis of ecological data segment analysis DCA (Detrended Correspondence Analysis) and canonical correspondence analysis CCA (Canonical Correspondence Analysis). We consider species Cirsium arvense, Tanacetum vulgare, Salix triandra, Salix alba, Anthriscus sylvestris as problematic for the operation of photovoltaic power plant. These species may be difficult for operation of the power plant if the maintenance is not regular.
EFFECT OF NITROGEN NUTRITION ON YIELD, OIL CONTENT A ND OIL PRODUCTION OF OILSEED RAPE (BRASSICA NAPUS L.)
VARENYIOVA MARIA, DUCSAY LADISLAV
view abstract xvarenyiova@uniag.sk

Abstract: The aim of the experiment was monitoring importance of nitrogen in nutrition of oilseed rape (Brassica napus L.) as well as the effect of rising doses of nitrogen on yield, oiliness and oil production of rapeseed. The plot–scale experiment was based in experimental year 2013–2014 in terms of agricultural cooperative in Mojmírovce. The experiment consisted of four treatments of fertilization. The block method of experimental plot size of 600 m² in three replications was used in this experiment. Hybrid Artoga was seeded and fertilized by different doses of nitrogen (160 kg.ha^-1, 200 kg.ha^-1, 240 kg.ha^-1). The lowest average yield 3.41 t.ha^-1 of rapeseed was noticed at unfertilized control treatment 1. The highest average yield 5.43 t.ha^-1 was reached at treatment fertilized by dose of nitrogen 200 kg.ha^-1N. The highest average oil content 47.41% was found at unfertilized control treatment. T he lowest average oil content 45.91% was found at treatment where the dose of nitrogen 160 kg.ha^-1was applied. The highest average oil production 2.49 t.ha^-1 was observed at treatment 3, where also the highest yield of seeds was found. These findings indicate that the highest dose of nitrogen does not result in the highest yield of rapeseed as well as there is not the inverse correlation between increasing doses of nitrogen and oil content, in this experiment.
EVALUATION OF VEGETATION ON LANDS WITH PHOTOVOLTAIC POWER PLANTS
VESPALCOVA TEREZA, CHOVANCOVA SVETLANA, WINKLER JAN
view abstract fulltext xvespal1@node.mendelu.cz

Abstract: The aim of this paper is to evaluate species structure of weeds on lands with photovoltaic power plant in Moravsky Krumlov. The observation was carried out on twelve test spots also under photovoltaic panels and between them. Vegetation was evaluated via phytocoenology relevé. The evaluation took three months (July, August, September) in 2013. The observation was statistically evaluated using DCA and CCA analysis. A total of 66 weed species were found on land with photovoltaic power plant. The largest coverage was noticed by species Poa pratensis L., Lolium perenne L., Achillea millefolium L. The most common specie between rows were: Plantago major L., Achillea millefolium L., Cerastium holosteoides Fr. The most occurring weed species under panels were: Poa pratensis L., Lolium perenne L., Festuca rubra L., Agrostis capillaris L., Bromus tectorum L.
POSSIBILITY OF SELECTION FOR HIGHER SEED VIGOUR OF BARLEY
VINTRLIKOVA EVA, KLIMESOVA JANA, STREDA TOMAS
view abstract fulltext xvintrl2@mendelu.cz

Abstract: Assessment methods for the seed germination are designed in an environment of ideal conditions, however, it is necessary to know the real behaviour in the field conditions. At this stage, on the series comes an important factor, which is based on the ability of germination – the seed vigour. The seed vigour is characterized ability by the seeds to emerge and create a basis for a new plant under real or stress conditions. The purpose of this study was to obtain general facts about the heritability of the seed vigour. Effect of drought stress on the observed characteristics of spring barley (Hordeum vulgare L.) was evaluated in the pot experiment. The root system size and seed vigour were evaluated in four genotypes. Seed vigour as the germination percentage under drought (-0.5 MPa) and temperature stress (10°C) was evaluated. It was also evaluated the relationship between seed vigour and the root system size of the parents and their progenies. Statistical significant correlations (r = 0.747–0.801) of the root system size in the stage of stem elongation and seed vigour in Variant III (moderate stress) were found. The root system size of parents at the stage of heading in unstressed variant (Variant II) statistically significantly (r = 0.730–0.939) influenced the length of the plumula and roots of the progenies in both variants of seed vitality testing (i.e. control and drought stress).
MONITORING OF LACCASE PRODUCTION BY FUNGAL ISOLATES FROM CZECH FOREST
VRSANSKA MARTINA, PALOVCIKOVA DAGMAR, VOBERKOVA STANISLAVA
view abstract fulltext martina.vrsanska@mendelu.cz

Abstract: Discovery of novel laccases with different substrate specificities produced by different fungal species is important for industrial, biotechnological and environmental applications. The aim of this work was to monitoring of laccase production by thirty five locally isolated white-rot fungal species. In five strains with the highest enzyme secretion laccase activity was examined using two different substrates (ABTS and syringaldazine) under different conditions (shaking, static). The measuring laccase activity using ABTS and syringaldazine as substrates confirmed that one of the best producers proved to be Fomes fomentarius and Trametes strains.