List of Contributions Conference MendelNet 2015Food Technology- THE MIGRATION OF PHTHALATES FROM PACKAGING INTO FOOD DEPENDING ON THE HEAT PROCESSING AND FAT CONTENT OF MEAT PRODUCTS
BOGDANOVICOVA SONA, JAROSOVA ALZBETA, MIHOK MICHAL, JANDASEK JOSEF
view abstract
fulltext
sona.bogdanovicova@mendelu.cz
Abstract: Phthalates (PAE) are organic lipophilic compounds mostly used as plasticizers to increase the flexibility of plastic polymers. Other applications include printing ink and varnishes. Humans are mostly exposed to phthalates via food; such exposure can have adverse effects on health. The goal of this study was to investigate the migration of phthalate compounds: di-n-butyl pthalate (DBP) and di-2-ethylhexil pthalate (DEHP) in model meat products of the Bologna type sausage category depending on the packaging used and the percentage fat content due to heat processing. - UTILIZING MALT FROM PURPLE WHEAT KONINI VARIETY FOR PRODUCTION OF TOP-FERMENTED BEER
DOSTALOVA YVONA, HRIVNA LUDEK, JANECKOVA MARIE, MACHALKOVA LENKA, MRKVICOVA EVA, VYHNANEK TOMAS, TROJAN VACLAV, PAVLU MILENA, JUZL MIROSLAV
view abstract
fulltext
yvona.dostalova@mendelu.cz
Abstract: This experiment is focused on the influence of malt produced from purple Konini wheat, which underwent kilning at 80°C, 100°C, and 120°C, on the resulting quality of the top-fermented beer. In the production of six different samples of beer we have used two ratios of barley (Pilsner) and wheat malt (50:50, 70:30). Individual samples were subjected to sensory evaluation including evaluation of colour. We have determined the extract content (actual and apparent), alcohol, and original gravity of the hopped wort. The highest alcohol content (5.25%) occurs in samples using light caramel wheat malt in both ratios of malt. The contents of both actual and apparent extract were increased to 6.5%, 4.8% resp., by using malt kilned at higher temperature (100°C or 120°C) and using a higher dose of wheat malt. Beers that show the lightest colour (over L* = 80) are those made with a greater proportion of barley malt and malt kilned at lower temperature (80°C). When comparing both ratios used, the better scoring assessed beers were those using more barley malt, therefore the best evaluated beer was produced from "Pilsner" malt type with 30% wheat and 70% barley malt. - USE OF COLOUR VARIETIES OF WHEAT IN THE BAKERY INDUSTRY
JANECKOVA MARIE, HRIVNA LUDEK, MACHALKOVA LENKA, DOSTALOVA YVONA, MRKVICOVA EVA, VYHNANEK TOMAS, TROJAN VACLAV, PLUCAROVA DANA, NEDOMOVA SARKA
view abstract
fulltext
xjaneck5@mendelu.cz
Abstract:Coloured wheat represents an interesting raw material for the food industry not only in terms of new products, but also for its positive effects on human health. We have tested four varieties of coloured wheat – Konini, Rosso, Scorpion, and UC66049. From the milling fractions obtained by milling grains of coloured wheat, we have compiled 11 pastry recipes. The results of sensory analysis indicate that the best variants for preparation of pastry recipes are variants 2, 9, and 10. Variant 2 contained only flour milled from the Konini variety. Variants 9 and 10 contained an admixture of 10% of bran particles. Variant 9 was produced from the Rosso flour variety and variant 10 from the Scorpion variety. - YIELD AND TECHNOLOGICAL QUALITY OF SUGAR BEET AFTER EXTRARADICAL NUTRITION
MACHALKOVA LENKA, HRIVNA LUDEK, HERNANDEZ KONG JOANY LIZET, STAVEK ONDREJ
view abstract
fulltext
lenka.machalkova@mendelu.cz
Abstract: This small-plot field experiment was aimed at testing the effects of extraradical nutrition on the quality of sugar beet production. The experiment also included the monitoring of root growth dynamics and changes in the root’s technological quality. The growth of taproots during the vegetation period corresponded with the development of the weather conditions. The sugar content in the taproots gradually increased up to an average harvest value of 18.1%. When monitoring the variants, the content of alpha-amino nitrogen did not undergo significant changes, staying in the rather positive values of 15–20 mg·100g-1. Similarly positive was the low content of noxious potassium at the point of harvest. The best results were obtained after repeated application of the Carbon Si fertilizer. This variant reached the highest taproot yield, polarization sugar yield and refined sugar yield per hectare. The highest sugar content was reached after repeated application of the Carbonbor Zn, Cu, S spray in combination with Insenol. The experiment has shown that extraradical nutrition promotes taproot yield and has a positive influence on technological quality as well. - QUALITY PARAMETERS AND CHEMICAL COMPOSITION OF COLORED-GRAIN WHEAT AFTER FOLIAR FERTILIZATION
MACHALKOVA LENKA, HRIVNA LUDEK, JANECKOVA MARIE, DOSTALOVA YVONA, MRKVICOVA EVA, VYHNANEK TOMAS, TROJAN VACLAV
view abstract
fulltext
lenka.machalkova@mendelu.cz
Abstract: Winter wheat varieties "Rosso" and "Skorpion" were cultivated in 2013–2014 in small-plot field trials. A half of variants were fertilized only with nitrogen as part of regeneration and production fertilization, while the other part was fertilized with sulfur and nitrogen. The total dosage amounting to 184 kg of nitrogen per hectare and 94 kg of sulfur per hectare. Accordingly, the qualitative fertilization involved NP solution, YARA Vita Thiotrac and combinations of both applied by foliar spraying. The application of sulfur combined with nitrogen or qualitative fertilization had no influence on grain yield. The qualitative fertilization increased the protein content by up to 0.8% for "Rosso" and only by 0.2% for "Skorpion", while the value of Zeleny sedimentation volume grew by 2.7 to 6.3 ml for "Rosso" and by 1 to 1.7 ml for the "Skorpion" variants fertilized with N1S1. The representation of individual protein fractions was significantly influenced only by the variety - no fertilization effect was demonstrated.For "Rosso", the content of selected cyanidins increased by l.0%–81.0% after the application of qualitative fertilization, while the influence for "Skorpion" was less significant, ranging from -9.3% to 37.3%.The extremely favorable conditions in the given crop year significantly eliminated the effects of the qualitative fertilization applied. - EFFECT OF GOAT MILK ANALYTICAL PROPERTIES ON ITS VISCOSITY AND CONDUCTIVITY
PYTEL ROMAN, KUMBAR VOJTECH, NEDOMOVA SARKA, SUSTOVA KVETOSLAVA
view abstract
fulltext
r.pytel@seznam.cz
Abstract: In this paper were analysed viscosity and conductivity of individual samples of goat milk. Milk samples were characterised by chemical analysis such as content of fat, dry matter, protein content, lactose, and titratable acidity. Viscosity was studied using a concentric cylinder viscometer. Results of milk samples viscosity were in range from 1.634±0.166 to 1.850±0.167 mPa∙s. The range of conductivity results was from 0.377 to 0.445 S∙m-1. Viscosity of goat milk was significantly depended on content of fat, proteins, and/or dry matter. Increasing titratable acidity of goat milk caused its conductivity increase. However, other parameters such as content of fat, proteins, lactose, and/or dry matter lead to decrease of goat milk conductivity. - THE LOAD ON THE SOILS IN THE CZECH REPUBLIC BY PHTHALIC ACID ESTERS
SIATKOVA MONIKA, JAROSOVA ALZBETA, POLAKOVA SARKA
view abstract
fulltext
xsiatkov@node.mendelu.cz
Abstract:The aim of the study was to monitor two esters of the phthalic acid, dibutyl phthalate (DBP) and di-2-ethylhexyl phthalate (DEHP) in agricultural soils in the Czech Republic in 2014. The concentration of DBP and DEHP was determined using a high-performance liquid chromatography (HPLC) with UV detection. The extraction was performed ultrasonically by a mixture of acetone:hexane (1:1). DBP and DEHP monitoring was performed in 12 regions of the Czech Republic. The DBP values ranged from 0.08 (Hlízov) to 1.78 mg.kg-1 (Chrlice) of dry matter. The DEHP values ranged from 0.01 (Hlízov) to 2.32 (Malenovice u Zlína) mg.kg-1 of dry matter. The sum of the concentrations of DBP and DEHP ranged from 0.09 (Hlízov) to 3.21 mg.kg-1 of dry matter (Chrlice). - EFFECT OF FISH OIL IN THE DIET OF THE MODEL ORGANISM ON HEMATOLOGICAL PARAMETERS AND CHEMILUMINISCENCE OF LEUKOCYTES
SKULTETY ONDREJ, KOMPRDA TOMAS , VALOVA MARKETA, ROZIKOVA VERONIKA, SUSTROVA TEREZA, FALDYNA MARTIN, LEVA LENKA, KAVANOVA LENKA
view abstract
fulltext
ondrej.skultety@mendelu.cz
Abstract: The purpose of the present study was to assess the effect of diet enriched with 2.5% fish oil (polyunsaturated fatty acids source) and the effect of diet enriched with 2.5% palm oil (saturated fatty acids source) to the overall health status of the model organism (Sus scrofa f. domestica). To determine the overall health status of the model organism, following hematological indicators of blood were analyzed: number of white blood cells, number of red blood cells, level of hemoglobin and hematocrit. There were non-significant differences in the investigated parameters of white and red blood cells, hemoglobine and hematocrit between groups of animals fed respective diets at day 29. No clinical signs of disease were observed during the entire experiment and hematological analysis gave results within the reference range, that gave evidence of the animals being in a good state of health. The level of oxidative stress of organism was measured via chemiluminiscence of leukocytes. There was no difference between fish oil diet and control group in the level of integral intensity of spontaneous CL as well as after stimulation by Zymozan. But the level of integral intensity of activated CL by PMA was increased by diet enriched with 2.5% fish oil compared with control group fed with diet enriched with 2.5% palm oil. Fish oil probably created oxidative stress in organism and antioxidants (in our case tocoferol) presented in feed were not able to avoid oxidative reaction of double bounds in the molecules of fish oil. - EFFECTS OF FISH OIL DIET ON M1 AND M2 MONOCYTE DERIVED MACROPHAGES POLARIZATION
SUSTROVA TEREZA, VICENOVA MONIKA, LEVA LENKA, ONDRACKOVA PETRA, FALDYNA MARTIN, KOMPRDA TOMAS, SKULTETY ONDREJ, SLADEK ZBYSEK
view abstract
fulltext
terez.sustrova@seznam.cz
Abstract: The aim of this study was demonstrated effect of fish oil diet on M1 and M2 polarization of macrophages. Six piglets were fed with standard diet supplemented with 2.5% fish oil containing eicosapentaenoic acid (EPA) and six piglets were fed with addition 2.5% palm oil as control group. We obtained mononuclear fraction of white blood cells from peripheral blood and we subsequently obtained CD14+ monocytes by magnetic separation. After 7 days of cultivation we obtained monocyte-derived macrophages (MDMF). It was measured genes expressions of pro-inflammatory soluble factors (IL-1β, TNF-α and MMP12) and anti-inflammatory (IL-10 and TIMP1) for detection of M1 or M2 polarization of MDMF. In the diet with fish oil, it showed a statistically significant increase in gene expression of MMP12 (P<0.01). It was measured genes expressions after stimulation of lipopolysaccharide (LPS). In case of both diet (fish oil and palm oil) IL-1 gene expression was increased in contrast to HPRT-1 (housekeeping gen). It is obvious that MDMF were directed to M1 polarization in fish oil diet. After LPS stimulation were both group of MDMF polarized as M1 – pro-inflammatory. - BIOCHEMICAL PARAMETERS OF BLOOD PLASMA AND FEED CONVERSION RATE DEPENDING ON THE DIET IN THE MODEL ORGANISM
VALOVA MARKETA, KOMPRDA TOMAS, ROZIKOVA VERONIKA, SKULTETY ONDREJ, TRCKOVA MARTINA, GOPFERT EDUARD, LORENCOVA ALENA, LEVA LENKA, FALDYNA MARTIN
view abstract
fulltext
market.valova@email.cz
Abstract: The aim of the present study was to determine the effect of diet enriched with 2.5% fish oil (polyunsaturated fatty acids source) resp. 2.5% palm oil (saturated fatty acids source) during the feeding experiment to the overall health status of the model organism (Sus scrofa f. domestica). Biochemical indicators of blood (alanineaminotransferase, aspartateaminotransferase, alkaline phosphatase, urea, total cholesterol, HDL-fraction and LDL-fraction) and feed conversion rate were analyzed to determine the overall health status of the experimental animal. Diet enriched with 2.5% fish oil significantly decreased (P<0.05) aspartateaminotransferase and alkaline phosphatase, but there was no change (P>0.05) in alanineaminotransferase. Diet enriched with 2.5% palm oil significantly decreased (P<0.05) aspartateaminotransferase, alkaline phosphatase and also alanineaminotransferase. Both diets decreased (P<0.05) level of total cholesterol, although we expected a reduction only in the diet enriched with fish oil and increasing in the diet enriched with palm oil. The level of HDL-fraction was increased (P<0.05) in the diet enriched with palm oil, but not in the diet enriched with fish oil (P>0.05). The level of LDL-fraction was decreased (P<0.05) in both diets, which was expected in the diet enriched with fish oil, but not with palm oil. The level of urea was decreased (P<0.05) in both diets. The effect of diet enriched with 2.5% fish resp. palm oil to the feed consumption, body weight gains and feed conversion rate was tested - there were not significant differences (P>0.05) between two experimental diets. These are only preliminary results obtained during the experiment, which are so far unclear and ambiguous, therefore further research is needed in this area. - A COMPARISON OF BIURET, LOWRY AND BRADFORD METHODS FOR MEASURING THE EGG’S PROTEINS
VRSANSKA MARTINA, KUMBAR VOJTECH
view abstract
fulltext
martina.vrsanska@mendelu.cz
Abstract: Quantitation of the total protein content in a sample is a critical step in protein analysis. Molecular UV-Vis absorption spectroscopy is very efficient in quantitative analysis such as protein quantitation and has extensive applications in chemical and biochemical laboratories, medicine and food industry. Traditional spectroscopic methods are cheap, easy-working and the most common way to quantitate protein concentrations. This study compares Biuret, Lowry and Bradford methods for measuring hen albumen and egg yolk as protein samples. These methods are commonly used for determination proteins. The Biuret test uses as a reagent: Biuret reagent. For Lowry assay are used four reagents: reagent A, reagent B, reagent C and reagent D. For last method, Bradford, is used as a reagent Coomassie Brilliant Blue G-250. The absorbance was measured at a wavelength of 750 nm for Lowry, 540 nm for Biuret and 595 nm for Bradford assay. The lowest content of proteins was analysed in albumen (0.706 mg·ml-1) and egg yolk (0.996 mg·ml-1) for Biuret method. According to the Lowry assay, was content of proteins in albumen 0.908 mg·ml-1 and content in egg yolk was 1.003 mg·ml-1. The highest content of proteins, which was analysed using method Bradford, was content of protein in albumen 1.125 mg·ml-1 and content for egg yolk was 1.369 mg·ml-1.
|
